Genetic Polymorphisms of GSTO1 gene and their association with the risk of Polycystic Ovary Syndrome in a sample of Iraqi Women

Abstract

Polycystic ovary syndrome (PCOS) is the most frequent endocrinological disorder, occurring in young women of reproductive age. The goal of this study was to look at a possible link between GSTO1 A140D (rs4925) polymorphisms and PCOS, investigating the effect of these polymorphisms on the levels of Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS). This study was carried out in the Laboratories of the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies - University of Baghdad during  the period from 1 November 2021 until the end of June 2022, The PCOS patients were taken from the Kamal Al-Samarrai infertility treatment Hospital in Baghdad. Women with PCOS (n=50) and  apparently healthy subject (n=50), were enrolled . Genotyping of GSTO1 gene SNP exon 4 (C to A; rs4925); was determined using Taqman genotyping assay by RT-PCR. The results showed that the distribution of genotypes and alleles frequencies at rs4925 C>A SNP of GSTO1 gene CC, and CA genotypes indicated no significant differences in frequency percentage which were noted between control subjects and patients with polycystic ovary syndrome. Whereas, the frequency of mutant AA genotype was  significantly (p<0.01) higher in patients with PCOS than in apparently healthy subjects(28% versus 6%, respectively, χ2=7.117, OR =1.67)this may be indicating a risk factor for the development of reproductive women to PCOS.