Molecular Detection of Neisseria Gonorrhoeae and Chlamydia Trachomatis in an Infertile Woman

Abstract

Background: The inability to conceive after a year of continuous, unprotected sexual activity is considered infertility, a reproductive condition that affects both men and women.

Aim: In the current investigation, bacterial vaginosis was isolated and identified using the real-time PCR technique to determine the influence of C. Trachomatis and N. Gonorrhoea infection as a risk factors for female infertility.

Methods: The research comprised 100 female infertility patients hospitalized in the hospital between March and June of 2022. One hundred samples of cervical swabs were collected. The sample was immediately kept at -20c until it was used at a later time to extract DNA.

Results:  After the DNA was extracted from one hundred samples of infertile women to detect and quantify Neisseria gonorrhoeae using the Real-Time PCR technique using specific target sequences primers for the (16S rRNA gene 132 bp), the result shows that only 30 samples gave a positive result for N. gonorrhea. Also, to detect and quantify Chlamydia trachomatis using Real Time PCR technique  using specific target sequence primers for the (16S rRNA gene 146 bp), the result shows that only 22 samples give a positive result for C. trachomatis.

Conclusion: Comparing RT-PCR to other approaches, it had