Determination of the Lethal Dose of Ultraviolet Rays on The Growth of Some Human on Pathogenic Bacteria

Abstract

Aim: The important time to stop the growth of some types of bacteria that cause human diseases by using UV- light (500nm), which reduces the effort and danger to health care workers. Background: In this study, we included the effect of ultraviolet UV- light (500nm) on some types of bacteria such as Staphylococcus aurous, Klepselia pneumonia, Acentobacteria that cause human diseases. Staphylococcus aureus is a Gram-positive bacteria and causative agent of wide range of infectious diseases such as skin infections, bacteremia, endocarditis, pneumonia and food poisoning. K. pneumoniae now commonly occurs in healthcare facilities, such as hospitals, and is responsible for a range of serious infections involving the urinary tract, lungs, abdominal cavity, intra-vascular devices, soft tissues surgical sites and causing bacteraemia, Acinetobacter baumannii can cause infections in the blood, urinary tract, and lungs (pneumonia), or in wounds in other parts of the body. It can also “colonize” or live in a patient without causing infections or symptoms, especially in respiratory secretions (sputum) or open wounds. Method: The study included 50 samples (swab) of S. aureus taken from people suffering from burns and wounds. And 50 samples (intestinal swab) were taken from patients infected with_ Acinetobacter and 50 samples (sputum) from patients infected with K. pneumonia Samples were collected from Al- private International Hospital in Baghdad _ during the period 11/15/2021 to 11/20/2022. All of these samples were .diagnosed by a medical staff using a (fine) device and agricultural media The samples were preserved with glycerin to be preserved for a longer _ period and used for conducting experiments, and at the same time the samples .were used as a (control).From the same tube containing the sample, a percentage (0.5) was taken) _ to conduct experiments, and the rest was used to conduct other experiments _ Using a dose of (UV and shedding it on the growth of some types of pathogenic bacteria). Result: After preparing plankton, some types of pathogenic bacteria are: S. aureus, Acinetobacter,K. pneumonia. A working method is prepared for applying UV doses to bacteria at different intervals of time: (10, 30, 60) minutes. It was concluded that time is important to know the time period in which the growth of each of the S. aureus bacteria whose growth stopped by a period (30 minutes,60 minutes), K. pneumonia, which stopped growing by a period (60 minutes), and the Acinetobacter by a time period (60 minutes) is stopped. Conclusion: Time periods were determined to stop the growth of each of the bacteria (Staphylococcus aurous - Klebsiella pneumoniae - Acinetobacter) after exposure to ultraviolet rays with a wavelength of (500nm). As the S. aurous bacteria stop its growth for a period (30-60 min), the - Klebsiella pneumoniae bacteria stop its growth for a period of time (60 min), and the Acinetobacter) bacteria stop its growth for a period of time (60 min).