Toxicity Assessment of Cobalt Ferrite Nanoparticle on Cancer Cell Lines

Abstract

Cobalt ferrite Nanoparticles (NPs) considered as promising biomedical tools in cancer treatment. Our purpose was to investigate the cytotoxicity of cobalt ferrite on mammary carcinoma cell lines CAL-51, AMN-3 and REF as normal cell lines by MTT (Methyl Thiazolyl Tetrazolium) and crystal violet technique. The reduction in cellular growth was concentration dependent. The inhibitory concentration (IC50%) after 72 hrs of incubation with a concentration 25 μg/mL CoFe2O4nanoparticles were 50%, 50.5%, on CAL-51, AMN-3 cancer cell lines respectively by MTT assay. The anti-tumor potentials of biogenic CoFe2O4nanoparticles which confirmed by using crystal violet method was significantly decreased in concentration 25 μg/mL CoFe2O4nanoparticles when reviewed microscopically after 72 hrs of exposure as compared to (REF) normal cell line. However, the morphological changes of CoFe2O4nanoparticles - treated cells exhibited apoptotic-like characteristics such as shrinkage and loss of normal fusiform appearance Furthermore, cells got separated from surfaces and from one another.