Molecular Detection and Diagnosis of Pathogenic Escherichia Coli and Giardia Lamblia of Patients in Babylon Province

Abstract

In this study detected and diagnosis the parasites (Giardia lamblia) and Escherichia coli Bacteria causes of diarrhoea according molecular, morphological examination and biochemical test. intestinal parasite spread in different regions of the world, especially in the tropical and subtropical regions, more than any region in the world because the population density increase beside temperate and cold areas that few take into account public health rules as well as the ability of a number of types of insects like flies on the transfer of cyst and egg of different parasites. A total of (96) stool specimens were taken from patients with diarrhoea and vomiting, during the period from February to June (2022) admitted to the Al-Hillah Hospital, Al- Imam Al-Sadiq Hospital and laboratories out. Among them 2 (2.08%) of specimens were found to be with G.lamblia and 25 (26.04%) with E. coli pathogens. In the present study, In the current study, the polymerase chain reaction (PCR) technique was also used, through which 96 samples were examined for microscopic examination of the parasites that cause diarrhea (G.lamblia ), and the infection rates was (2.08%%) ,As for the bacteria, 96 samples were examined for the bacteria that cause diarrhea( E.coli), and the infection rates was (26.04%). Depending on the PCR technique, the highest rates of infection was shown in males (39.2%), while the infection rates in females decreased (35.5%) for parasites. As for bacteria, the study recorded an increase in the infection rates in females (31.7%), while the rates of infection decreased Infection in males (21.81%), and this study recorded the highest infection rates in the age group (6-10 years), reaching (50%), while the decrease in the infection rates was in the age group (21 years and over) and was (10%) for parasites. As for bacteria, the study also recorded an increase in the rates of infection in the age group (6-10), reaching (35.29%), while the study decreased in the age group (21 years and over), reaching also (10%).