Biosynthesis and Antioxidant of Gold Nanoparticles by Endophyte Fungus Aspergillus Fumigatus Isolated from Sanna Surattensis Leaves

Abstract

In the modern era, the fabrication of nanoparticles is anew and advanced approach in the field of nanotechnology. Studies that include the description of gold nanoparticles produced by endophyte microorganism are important and novel in the field of technology, especially. In this study Aspergillus fumigatus was isolated from the healthy leaves of Sanna surattensis plant as endophyte fungus. The fungal isolate was identified by using advance molecular approach.GDNA was isolated, the target fragments of 18S rRNA amplified and send for sequencing following .A.fumigatus cell-free filtrate was used in the biosynthesis of gold nanoparticles (AuNPs) via reduction of gold salt (HAuCl4)solution to nanocrystal. The formation of AuNPs was monitored by observing the color change. The UV-VIS spectrum confirmed the reduction of gold ions. The resulting nanoparticles were characterized by Transmission electron microscopy(TEM) and X-ray diffraction (XRD).AuNPs were synthesis using A.fumigatus filtrate and tested for free radical scavenging activity by 1,1- diphenyl 2-picrylhydrazyl (DPPH) method .The size and shape of AuNPs determined by TEM were mostly spherical, and the average size 23 nm. The result emphasized of (XRD) indicates that the resulting material is pure gold nanoparticles. AuNPs stated significant antioxidant activity was comparable to the standard ascorbic acid.