Different concentrations effect of indocin medicine on some biochemical and physiological blood parameters in pregnant white rats.

Abstract

The current study was carried out in the department of biology at the college of Education for Girls - University of Kufa to measure some blood parameters (biochemical and physiological) of pregnant white rats treated with indocin medicine at doses of (8) mg/kg of body weight during the two gestation stages 14th day and 20th day respectively. The total number of rat animals used in the current study was 40 white female rats aged 11.5 weeks and weighing 229 g and 40 fertile white male rats aged (11) weeks and weighing 224 g,the female and male rats were married for the purpose of obtaining of required number of pregnant female rats after that the pregnant animals were divided into two groups: the first group contained 40 pregnant rats treated with indocin at a concentration of 8 mg/kg of body weight during 14th day to 20th day of gestation to study some biochemical variables which were represented by (Albumin, Erythropoietin,Vitamin D3, Calcium ions and Malondialdehyde (MDA)) and some physiological variables which were (RBC count, concentration of hemoglobin (HB) and value of PCV). The total pregnant female rats were divided into two groups, the first group was the control group and were dosed with physiological salt, while the second group was treated with indocin medicine at a dose of 8 mg / kg of body weight, the pregnant rats in two groups were treated from 1st day of gestation daily with a tube of oral administration of rats,after the treatment, 10 pregnant rats were dissected during 14th day of gestation from each group, while the remaining 10 pregnant rats were dissected during 20th day of gestation from each group,The the calculations of the study results revealed a significant decrease (P<0.05) in the concentrations of albumin, erythropoietin and vitamin D3, RBC count, concentration of hemoglobin (HB) and value of PCV, while there was a significant increase (P<0.05)in the concentrations of malondialdehyde (MDA) and calcium ions in the serum in rats of groups treated with indocin at a dose of 8 mg/kg during gestation of 14 and 20 days respectively when compared with control groups during the same two stages of gestation (14th day and 20th day) respectively. Conclusion of this study was that indocin medicine caused abnormal effects on the concentrations of some biochemical and physiological blood parameters including albumin, erythropoietin, vitamin D3, calcium ions and malondialdehyde(MDA), RBC count, concentration of hemoglobin (HB) and value of PCV of pregnant female rats during two gestation stages 14th day and 20th day respectively and these effects increased with increasing gestation stage.