Assessment of Interleukin -6 and Interleukin -10 in Patients sera of Urinary tract Infection

Abstract

A urinary tract infection (UTI) in humans is one of the most common ailments in developing countries. The treatment of UTI is becoming difficult because of the increasing drug resistance against the common bacteria associated with UTI. This research aimed to determine the bacteria and to detect the host cytokine response to UTI infection. A retrospective study of data ( November 2020 through May 2021) from the microbiology laboratory of the General Baghdad Hospital was analyzed. Bacteria were isolated from 250 patients (43.88%) urine cultures of 139 UTI suspected urine samples. Both Gram-positive (Staphylococcus aureus 24.5%; S.hominis 1.6%) and Gram-negative bacteria (Escherichia coli 62.2%; Klebsiella pneumonia 6.5 %; Pseudomonas aeruginosa 4.9% ).The level of IL_6 and IL-10 in patients sera were measured, the level of IL-6 and IL-10 increased significantly in UTI patients in comparison to healthy control. The level of IL-6 and IL-10 differ significantly in UTI patients sera according to their interval ages.,the highest level was at ≥ 30,when the mean± S.D of IL-6 and IL-10 reached to 77.75 pg/ml ± 8.62 for IL-6 and 233.66 pg/ml ±17.56 for IL-10, While the less level was at ≤ 50. The level of IL-6 and IL-10 differ significantly (p≤ 0.05) in UTI patients sera according to gram positive and negative bacteria. When it gave mean level of IL-6 ± S. E in infection with S.hominis (200 pg/ml ± 0.03) higher than other Gram positive bacteria ( 67.56pg/ml ± 7.82). compare with Gram negative bacteria,it gave mean level for IL-6 In serum ± S.E with K. pneumoniae was 92.90 pg/ml ± 37.52.higher than E.coli and P.aerugenosa (72.45± 12.75 ) and (38.18± 0.16) respectively.