Serum levels of IL-23, IL-8 and IL-10 to CMV, HHV-6, and IBV infection and Male Unexplained Infertility

Abstract

Back ground: Infertility is a reproductive disorder  After 12 months or longer of frequent sexual intercourse Infertility affects 60–80 million people worldwide. Unexplained or idiopathic infertility is a condition, in which couples are not able to conceive without any definite causes. Viruses, as well as many other microorganisms, are able to interfere with the reproductive function and suspected to be involved in  development to infertility .Cytomegalovirus (CMV), human herpes virus type 6 (HHV-6) and Epstein–Barr virus (EBV) are also frequently discovered in sperm samples. Pro-inflammatory cytokines like IL-8 and IL-23 have direct effects on spermatogenic cell differentiation, testicular steroidogenesis, and spermatogenesis,  Whereas anti-inflammatory cytokines like IL-10 are also linked to testicular development

Aim of study : To detect the levels of interleukin  -23,IL-8 and IL-10 levels  as immune response’s for Un explained infertile men infected byCMV,HHV-6and EBV.

Materials and methods:  blood sample (3.5) ml taken from  twenty five patients of unexplained infertile males Herpes viruses ( CMV ,HHV-6and EBV)  with 20 appearance healthy fertile male  as control  in Iraq's Diyala province were studied From November 2020 to April 2021 measurement for them the  Interlukin-23, IL-8, and IL-10 were measured by   using an ELISA kit from My Bio Source in the United States. The statistical analysis was carried out using the software GraphPad Prism version 3.06 and SPSS version -26.

Results: In this study  we observed that  IL-23 in positive infertile male infected by CMV had  significantly higher  of IL-23 in plasma than CMV-seronegative recipients (P = 0.001),its respectively( 942.57±205) and (714.5±35).   Infertile men positive to EBV had IL-23 (951.51±432) while not infective by EBV (793±148) , no  significant different appear between them . IL-23 was male (924.9±227) in unexplained infertility men positive to HHV-6 ,not infective  males by HHV-6 (775.9±148) ;no  significant different  between the level of IL-23 in study group. Chemokine IL-8 mean of infertile males who positive to CMV was (213.762±64) and while negative results to CMV( 118.9±49) with high significant relation  between positive and negative  results  ,IL-8 in infertile male had  EBV (222.85±75) and where is  non infective unexplained   infertile males mean  (151.12±71), With no significant different .IL-8 arising in male infertile  with positive result to HHV-6 (221.9±63) and lowered in negative HHV-6 (140.6±66),with high significant different between positive and negative results in patients.Anti- inflammatory interleukins-10 found in  serum of infertile males  positive CMV (152.25±46) and negative CMV infected males (88.8±21) ,with significant different.  EBV had IL-10 mean (137.79±45)  in infertile male while  not infected by EBV had (112.12±46),no significant different appear between them .IL-10  in unexplained male positive result to HHV-6 was higher level form those who had negative result to HHV-6,  and  its (153.1±45) ,(104.4±41) respectively .with higher significant different appear between them ,no one of control  infected by HHVs  study in thes investigation and appear lower level of interleukins’  than infertile and there mean was for IL-23 (645.2±37),for  IL-8 was (86.4±10) and IL-10 was (    75.1±9). In conclusion, this study found most infertile male had showed a significant increase in IL-23,IL-8 and IL-10  to infection by human herpes virus (CMV ,HHV-6and EBV ) because these virus  could modulated immune system and there cytotoxicity that causes rising of expression of cytokine .