Association of Chymase and Angiotensin II with Early Detection of Diabetic Nephropathy in Patient with T2DM

Abstract

Diabetes mellitus(DM) is a metabolic disease with the mean characteristic is hyperglycemia resulting from defects in insulin action, insulin secretion, or insulin resistance, it always jointed with long-term harm, dysfunction, and failure of organs, particularly the heart, nerves, eyes, kidneys, and blood Objective:- This study aims to investigate the association between chymase and angiotensin ll levels in type 2 diabetic patients with early and chronic diabetic nephropathy.Subjects and Method:- A case-control study of individuals with type 2 diabetes was conducted from November 2021 to May 2022. This study was conducted on 62 patients with type 2 diabetes who attended Imam Hussein Medical City in Karbala. And 60 subject as a healthy control group. The patients were divided into three groups according to the ratio of their urinary albumin to creatinine ratio (ACR): Normoalbuminuria (n=22) the ACR was less than 30, Microalbuminuria(n=20) the ACR (30-299) and Macroalbuminuria (n=20), ACR was greater or equal to 300. All biochemical parameters measured by spectrophotometer, chymase and Angiotensin II measured by ELISA. Result: - The study of the Comparison between type 2 Diabetes Mellitus with Diabetic Nephropathy groups for Biochemical Parameters showed In (HDL, LDL, FBS, CHOL, HbA1c, Macroalbuminuria, TG, VLDL, blood urea, serum creatinine, and Angiotensin II), there were statistically significant differences between all groups, as well as in the Chymase it was statistically significant differences between the first and third and second and third groups.The results showed that there was a significant positive correlation between the blood levels of Microalbuminuria and ACR Opposite Chymase, and ANGII levels of the patients. Conclusion: - Increased levels of both chymase and ANG II levels play a role in the pathogenesis of diabetic nephropathy, the current study demonstrates that higher HbA1c% and poor glycemic control are closely associated with a greater likelihood of increased levels of chymase and ANG II and thus increased risks and complications of diabetic nephropathy in patients with type 2 diabetes mellitus.