The prevalence of TNF-α polymorphism in an Iraqi pediatric population

Abstract

Background: Many polymorphisms in the TNFα promoter gene had been reported. The G/A polymorphism at −308 has been found to be correlated with a 20%-40% increase in TNF-α production. Objective: To study the prevalence of TNF-α rs3093661 genotype in an Iraqi pediatric population. Materials and methods: Amplification-refractory-mutation -system (ARMS-PCR) technique was used to identify this genotype in all 96 subjects. Results: The genotypes of the targeted SNP were distributed as following: (rs3093661): 21 (21.9 %) AA genotypes, 19 (19.8 %) G/A genotype and 56 (58.3 %) GG genotype. Conclusion: The distribution of TNF-α (rs3093661) Polymorphism was detected by ARMS-PCR technique. At this locus there are three genotypes: GA, AA and GG. The wild type of homozygote genotype was showed only G allele amplification at 173bp product size. The mutant type of homozygote genotype was showed only A allele amplification at 173 bp product size. Whereas the heterozygote genotype was showed G and A alleles amplification at 173 bp product size respectively.