The antioxidant activity of Vitamin C against Methimazole-induced hypothyroidism in rats

Abstract

The study was investigated the antioxidant activity of Vit C against methimazole-induced hypothyroidism, the study included 4 groups, group, will be given sterile saline (0.9%) and served as a control. Group II: given Methimazole at dose 0.04 mg/kg B.W. orally (positive control). Group III: given Methimazole at dose 0.04 mg/kg B.W. and Vitamin C 200 mg/kg B.W. orally.  The group : given only Vitamin C 200 mg/kg B.W. orally , in different times , Day 0, Day 20 and Day 40  then thyroid hormones (T3,T4), TSH  and  17 β-estradiol were determined , the results showed  the parameters were not changed in Day 0 but , T3 and T4 was higher in group 4  in Day 20 and 40 , However, group 2 and 3 but group 2 recorded the lowest value in in day 40  for T3 (1.75 ±0.145 ,1.47 ±0.226 and T4 (30.10 ±7.163, 20.07 ±7.404  respectively), while the higher TSH concentration was in group 2 and 4 in day 40 (0.19 ±0.294, 0.19 ±0.284 respectively) and lowest concentration in group 3(0.06 ±0.152), finally  the β -estradiol concentration was higher in group 4 in day 20 and 40 (159.00 ±3.511 , 140.71 ±3.199 respectively), while the concentration was lower group 2 in day 20 (153.83 ±2.041) and day 40 (133 ±1.414).Depending on the obtained results Vit C encountered the methimazole effect to induce hypothyroidism  in animals through return the hormones concentration to the normal range comparison with the control group.