HLA-G Gene Polymorphism and Soluble HLA-G Serum Level in Rheumatoid Arthritis Patients under Treatment with TNF-γ Antagonists

Abstract

Objective: - Aim of the study to investigate the association between HLA-G gene polymorphism and soluble HLA-G serum level with rheumatoid arthritis patients on biological drugs. Materials and Methods: - HLA-G (rs1063320) SNP was genotyped and measurement of serum level was done in 100 RA patients on biological treatment ( Etanerecept (Enbrel) or Infliximab) from 4 to 6 months  and in 100 healthy controls. HLA-G SNP was analyzed by SYBR Green-based quantitative real-time polymerase chain reaction (qPCR) allelic specific assay. Serum level of soluble HLA-G investigate by ELIZA technique. Results: - There was a significant association between the HL-G (rs1063320) SNP with risk to develop RA (P value˂0.001). Also, soluble HLA-G serum level was lower in patients with RA than control but statistically not significant (p value 0.121), but significant relationship with DAS28 p=0.01. Conclusion: - HLA-G (rs1063320) SNP may be risk factors to develop RA. Positive association between serum level of sHLA-G and DAS28.