Determination of Vitamins D3, E and C in a mixture by HPLC

Abstract

A new analysis method has been found for the determination of vitamin E, D3, and C in one mixture using HPLC technology and separation in a short time and high accuracy using a mobile phase that is easy to prepare and apply, does not contain a buffer solution and does not contain expensive materials. using column C18 (dimensions: 150mm*4.6mm, 5µm), The mobile phase consists of methanol only with a flow rate of 1.5 ml/min  with a UV detector at a wavelength of 280 nm, and the retention time of vitamins C, D3 and E was 0.909 min , 3.685 min and 4.972 min respectively, The method had an accuracy between 95.59475% - 102.604% with a linear range between 20-180 μg.ml-1 , 80-400 μg.ml-1 and 5–20 ng.ml-1, The correlation coefficient R2 9994, 0.9990 and 0.9996 , A limit of detection was 0.001101 ng/ml, 0.13532 ng/ml and 0.123338 ng/ml , A limit of quantification 0.003669 ng/ml , 0.451066 ng/ml and 0.411125 ng/ml for Vitamin C, D3, and E, respectively .