Prevalence of ESBL production among E.coli causing urinary tract infection

Abstract

Urinary Tract Infections (UTIs) caused by Escherichia coli (E.coli) producing extended-spectrum β -lactamase (ESBL) are more expensive to treat than ESBL negative E.coli. Estimation the prevalence of ESBL-production among urinary E.coli isolates is essential due to its great effect on the choice of proper antimicrobials. Accordingly, the aim of this study was to detect the prevalence of ESBL-producing E. coli isolated from urine samples in Kirkuk/Iraq. 150 midstream urine samples collected from different hospitals in Kirkuk city. E.coli isolated and identified by microscopical examination, biochemical test and vitek 2 system. Phenotypic detection of ESBL was performed using double disk synergy method, also genotypic detection of ESBL genes (CTX-M) was performed using PCR technique. In this study 40 E.coli was detected, the incidence rate of ESBL-producing E.coli was 34(85%) .Remarkably ,dissemination of blaCTX-M gene was 33 (82.5%) among E.coli isolates and the gel electrophoresis showed that the molecular weight of amplified gene was 321 bp .