Antibacterial Activity of Protease that Production, Purification and Characterization from Proteus Mirabilis against Gram Positive and Negative Pathogenic Bacteria

Abstract

This research was accompanied in the laboratories of Biology Section, Faculty of Science, that deal with production, purification and characterization of protease by Proteus mirabilis which carried out for better making of protease using 1% casein as the substrate of enzyme, the manufacture was carried out by flooded fermentation, the greatest surroundings were the isolated of protease in synthetic medium, it donated great titer of protease activity, cultivation period 48 h, cultivation temperature 30 °C and pH = 7, the protease was purified using precipitation by ammonium sulphate (60%) and dialysis, the developed protease had finest activity at pH = 7, the protease was stable with pH values ranging between (6 - 9) and in temperature 40 °C also protease was stable in (30- 50) °C,examines of the protease for molecular weight was approved out by SDS-PAGE electrophoresis which discovered 47 KDa, Among the 5 pathogenic bacteria tested only 2 isolates, Micrococcus sp. and S. aureus showed inhibition zone on protein pellete treated agar after 24 hour with inhibition zone 9 mm for Micrococcus sp. and 12 mm for S. aureus, but no antibacterial activity of protease showed against gram negative bacteria involved E.coli, Salmonella typhi,, and Pseudomonas aeruginosa