A New Colorimetric Method to Determine the Acetylcholine Esterase Mimic Activity of V2o5 Nanoparticles

Abstract

Acetylcholine esterase (AChE) is a primary enzyme from the family of enzymes called cholinesterase (ChE) localized in the cytoplasm, blood, and neural synapses which allowing to work on hydrolyzed acetylcholine to choline and acetic acid intracellular and extracellular, this response is important to enabling a cholinergic neuron to come back to its resting state after activation. We successfully used a simple and a new method (colorimetric) to determine the mimic activity of VPNs as acetylcholine esterase using the change in color at length wave 616 nm of the solution containing bromothymol blue as an indicator which depends on the change of pH solution result from the decomposition of acetylcholine (Ache) to choline and acetic acid. Our results found that all samples get acetylcholine esterase mimic activity, while the sample annealed at 500 oC has the highest activity (13.983 x 10-4 U. min 1) compared with other samples.