Study of Ceftriaxone Resistance Gene in Bacteria Isolated from Children with Nephrotic Syndrome

Abstract

Objective: This study aim to study bacteria isolate to from Nephrotic syndrome children have septicemia by detect isolated bacteria resistance genes to antibiotics common for children (Ceftriaxone).

Method: In the Pediatric teaching hospital Center for Nephrology /Karbala Health Directorate, a five -milliliter sample of venous blood was collected from 116 Nephrotic syndrome children patient and 42 healthy children in age (months to 15 years). There are significant blood culturing procedures that was followed, and several analyses performed, the resistant genes for ceftriaxone were identified from positive blood culture sample by PCR Technique.

Results: Bacteria isolated was Acinetobacter bumanii complex, Staphylococcus warnerii, and Bacillus cerus. All of them had resistance genes for β-lactamase drug. Acinetobacter bumani which resistance to ceftriaxone showed that blaSHv and blaCTX_M were found and appeared in band 795bp and550 bp respectively in this species of Acinetobacter bumani complex. Staphylococcus warneri which resistance to ceftriaxone showed that blaZ and was found and appeared in band 173 bp. Bacillus cerus which resistance to ceftriaxone showed that genes blaA1 was found and appeared in band 680 bp.