Using of Serological and Molecular Technique for Toxoplasma Gondii Diagnosis in Goat and Cow in Some Regions of Baghdad Governorate

Abstract

Toxoplasmosis is aworldwidezoonosis common disease, caused by Toxoplasma gondii. Goats and cows are important sources of infection for humans,which caused public health problems in many countriesdue to reproductive and economic result from abortion and neonatal mortality in goats, so the aim of this study was to conduct a Seroprevalence of Toxoplasmosis in Baghdad governorate to determine the incidence of infection and link it to risk factors. Serum samples were collected randomly from 50cows and 104 goats, from different areas of northeast of Baghdad in Iraq and T. gondiiantibodiestiter were detected by ELISA technique using kit from ID vet company (French origin) in Central Veterinary Laboratory.

The results of the serological examination showed that the infection rate in cows was 18% andin goats 60% .There was clear effect for gender, age, food and water source, and region on the infection rate.The results of the molecular examination, when it was conducted on samples positive for the ELISA test, showed that the rate of infection incows was 33.33% (3/9)and ingoats 45% (18/40) positivefor PCR technique, by targeting a specific part of the B1 gene.