Evaluation of the Impact of the WFS1 Gene Rs10010131 (A/G) Variant on the Incidence of Type 2 Diabetes in Iraqi Arab Patients

Abstract

Several recent studies have examined the association between a group of genes and the incidence of diabetes mellitus. Some of these have focused particularly on the link between allelic variations of the Wolfram syndrome gene (WFS1) and insulin secretion, insulin sensitivity, and the subsequent risk of hyperglycemia and type 2 diabetes (T2D). While it is obvious that WFS1 mutations induce diabetes, the genetic mechanism for the link between common WFS1 variations and T2D remains unknown. This study was to investigate the association between WFS1 gene polymorphism (rs10010131 A/G) and T2D in Iraqi Arab patients, and particularly whether these effects are more profound for obese patients than for other patients. A case-control study of 200 subjects (100 T2DM and 100 controls) was used to investigate the above relationships. Blood samples were collected and RT-PCR assay was used to genotype WFS1 rs10010131 A/G, making use of newly-designed primers. A significant difference between obese participants in the control and patient sample groups in terms of codominant genotype, dominant genotype, and additive genotype. In addition, TG and VLDL showed a significant difference between AA vs AG and AA vs GG, a negative correlation between F. G and LDL VLDL and between LDL, HDL, and TG, between insulin and HOMA-IR with LDL, HDL. WFS1 gene polymorphism (rs10010131) A/G is associated with a heightened risk of T2DM, particularly in obese T2DM patients in Iraqi Arab patients.