Quantitative Determination of A Steroidal Drug Using Reversed-Phase High- Performance Liquid Chromatography (RP-HPLC)

Abstract

Dexamethasone was quantified in a fast, accurate and sensitive way using Reversed-phase high-performance liquid chromatography (RP-HPLC) method.

In this method, a separation column of porous silica particles L3 (250 × 4.6mm) was used, the flow rate is 0.5 ml/min, the column temperature is 25 °C, the Reagent wavelength is 250 nm, and the injection volume is 20 μl. As for the mobile phase, it consisted of acetonitrile and buffer solution dipotassium phosphate and monopotassium phosphate (50:50 v/v) and the pH was 8. The method was validated by calculating the recoverability that ranged between (103.39-104.15%), and the correlation coefficient (0.9959-0.9964) and linearity (0.1-100 μg/ml) the method was successfully applied for the quantitative determination of the mentioned drug.