Detection of blaOXA-23 Gene Dissemination among Various Bacterial Pathogens Isolated from Urinary Tract Infection (UTI) in Al-Najaf Al-Ashraf

Abstract

This study aimed to explore the dissemination of bla_OXA-23 gene among bacterial causative agents of urinary tract infections. A total of 54 samples of urine from patients with Urinary Tract Infection (UTI) during the period from November, 2021 to March, 2022, out of 36 samples were positive growth. Gram-negative bacteria were 27 (75%) (Which include 11(31%) of isolates were identified as E. coli, 7(19%) of isolates identified as K. pneumoniae, 6(17%) Acinitobacter bumanni, 2(8%) proteus merabilis, and 1(3%) Pseudomonas aeruginosa.). While 9 (25%) were gram-positive isolates 6 (17%) were S. aureus and 3(5%) were Enterococcus faecalis. Carbapenems susceptibility was performed by disk diffusion method, where Meropenem antibiotic showed that out of 36 isolates 19 (55%) were resistant (6 (31.5%) A. bumanni, 3 (15.8%) S. aureus, 3 (15.8%) E. feacalis, 2 (10.5%) E. coli, 2 (10.5%) K .pneumoniae, 2 (10.5%) P. mirabilis and 1 (5.4%) P. aeruginosa), as for Imipenem antibiotic showed that out of 36 isolates 16 (45%) were resistant (6 (37.5) A. bumanni, 3 (18.7%) K. pneumonia, 3 (18.7%) S. aureus, 2 (12.5%) E. coli, 1 (6.3%) P. merablis and 1 (6.3%) P. aeruginosa.). Total DNA extract samples were used in         bla_OXA-23 gene detection, results showed that total  Acinetobacter baumannii  isolates(6) were positive for bla_OXA-23 gene, then in K. pneumoniae out of 7 isolates 4 were positive for bla_OXA-23 gene, out of 11 E. coli isolates 2 were positive for bla_OXA-23  gene, in Proteus mirabilis and pseudomonas auroginosa isolates 2 and 1 respectively all were positive for bla_OXA-23 gene, while in all isolates of S. aureus and  Enterococcus faecalis bla_OXA-23  gene was not detected. We conclude that various types of gram negative UTI causative agents harbored bla_OXA-23, meanwhile its absence in isolated gram positive isolates.