Human Adenovirus Type 5 Infection and Tumor Suppressor Factors

Abstract

Several promyelocytic leukemia proteins (PML) isoforms are involved in a wide range of cellular functions, including innate immunological responses, gene transcription, and apoptosis.  Adenoviridae are non-enveloped viruses that can disrupt cellular systems that control cell cycle progression and apoptosis, as well as those that affect the synthesis of mRNA viruses that cause cancer. The study analyzed the gene expression of IFN (A fused gene formed when pieces of chromosomes 9 and 22 break off and trade places), into cells transfected with adenovirus and PML expression plasmids. A human breast cancer cell line (MCF-7) cells were plated in 24 well plates in 500 microliters of Dulbecco's Modified Eagle Medium (DMEM) for each well, and after 24 hours, the cells were harvested and used in this study. The 24-well plate was split into six rows, which were as follows: Each 6-well row was challenged with 1.2 mL of Ad-5 plasmid, PML-II, Ad-5, and PML-II, DNA transfection reagent, only serum-free medium.