Genotoxic Effect of Arsenic Trioxide on Rats Treated with Alpha Lipoic Acid.

Abstract

This study was designed to identify and investigate the genotoxic effect of arsenic trioxide on the oncogenes cyclin D1 and p27 kip1 in treated rats with arsenic trioxide for this reason, three groups of rats, 2 weeks old, first treated with Arsenic trioxide (ATO) (1.5mg/ kg Bw), the second group treated with ATO and Alpha lipoic acid (ALA) (100 mg/ kg Bw) and 3rd group (control) treated with phosphate buffer saline (pbs) orally) and the experiment and treatment were continued for 3 monthes. The results showed that the 1st group received ATO demonstrated increase (p≤ 0.05) in cyclin D1 mRNA gene expression level (8.932926 ± 1.6916) as compared with control group (1±0) and a decrease in the expression level of p27 kip1   mRNA gene (0.167883 ±0.01620) compared with control. The 2nd group that received both ATO and ALA showed the opposite results a significant decreased in cyclin D1 m RNA gene expression level (4.135597 ± 0.6603) compared to the control group and an increase level (p≤ 0.05) of p27kip1 m RNA gene expression level (3.592233± 0.3266) compared with control group. Conclusion ATO have a genotoxic effect on rat tissue through increase expression level of cyclin D1 and decrease expression level of p27kip1 mRNA and the genotoxic effect of ATO was ameliorated by ALA with opposite result level of cyclin D1 and p27kip1.