Molecular Detection of Enterotoxogenic Bacterial Toxins of Shiga Toxin Prodveing Escherichia Coli Non 0157 Serogroup 026 Isolated from Human and Calves Stool

Abstract

The present study includes the molecular characterization of STEC non 0157: 026 toxins. The reliable isolation as non-O157 STEC serotyping by specific latex agglutination test for the target non-O157 STEC (big six) serogroup (O26, O45, O103, O111, O121 and O145), and then confined the serotyping by targeting wzx (O-antigen) for (wzxO26, wzxO45, wzxO103, wzxO111, wzxO121, and wzxO145) by using PCR, then the positive isolate analyzed to determine the prevalence of main virulent genes (stx1, stx2, eaeA, and EHEC hlyA) among isolated non-O157 STEC. The results depending on PCR showed the prevalence of non-O157 STEC were 20 of out 127 (15.73%) in sample collected from children and 27 / 133 (20.30%) in calves sample, The serotyping showed that the O26 was 10.23% (13/127) in human sample and 8.27% (11/133) in animal sample, O103 was only detected in calves samples with 2.25%(3/133), meanwhile the O111 give positive result in both humans and calves samples, 2.36% (3/127) and 3.75% (5/133) respectively, the O145 was detected as 1.57% (2/127) in humans samples and 1.50%(2/133) of calves samples. The O45 and O121 give a negative result in both species. STEC 026 was the most frequent serotype among the other non 0157 STEC, it showed positive result in PCR analyses to the following genes (wzx026, stx1, stx2, eaeA and  EHEC hlyA. Conclusion:  In conclusion non-O157 STEC were important for the infection of children and calves were played important reservoir for Non-O157 STEC. And the more common strain of Non-O157 STEC was STEC O26.