Trichosporon Asahii Used in The Production of Silver Nanoparticles with Anticancer Effects

Abstract

This study was conducted to evaluate the efficiency of silver nanoparticles anticancer activity which produced by T.asahii and characterization of the synthesized silver nanoparticles by UV- spectroscopy and scanning electron microscope. the resultes revealed the ability of T. asahii to produced silver nanoparticles. Within 2-4 hours, the reaction started, and coloration changed to a yellowish brown,also T. asahii was observed to produce well-dispersed, homogenous AgNPs with a variety of morphologies, the majority of which were spherical. A nanoparticle's average diameter was 43.17, and the size range of AgNPs was between ( 15.63-75.92 nm). The weight percentage of elemental constituents for AgNPs was 71.7, T. asahii average size of the nanoparticles generated from T. asahii was discovered to be 27nm . The resulting nanoparticles FTIR spectra reveal distanced twelve absorption bands ranging between 3453.99 to 461.79 cm-1, indicating the presence of twelve active functional groups. The results showed a significant inhibition of MCF-7 proliferation after 72 hrs, Dose and time depended cytotoxic cell was observed in AgNPs in the range of 6.25 -100 µg/ml concentration. AgNPs showed 100.0% of the cytotoxicity at the highest concentration (100 µg/ml) after 72 hrs. exposure while the lowest cytotoxicity showed at concentration 6.25µg/ml and 12.5µg/ml which were 72.2% and 73.4% respectively From the assay, the determined IC50 values for AgNPs were 72.2% .