Differential Pulse Polarographic Analysis of Ceftriaxone and Cefotaxime and Its Estimation in Pharmaceuticals

Abstract

Direct DPP identification of ceftriaxone and cefotaxime in pharmaceuticals has been made for the development and validation. Based on the data results, it was determined that a 4 mm3 mercury drop size at 25°C in an acidic medium with a citrate-phosphate buffer solution at pH of 2-4 and 1 ml of LiCl, 1 M and 0.5 ml of KCl, 1 M as a supporting electrolyte yielded the most accurate findings for analysing ceftriaxone and cefotaxime Ceftriaxone had two peaks, the primary one at - 0.67V and the second at -0.95V, also cefotaxime has two peaks at - 0.67V and at -1.11V. A standard graph of ceftriaxone also cefotaxime in the concentration ranging between 3–20 μg.mL-1 was created and applied for the estimation of unknown concentration. The precision as well as accuracy of this method for determining both analytes were evaluated. In laboratory samples of ceftriaxone, the SD was 0.0866 and the RSD% did not surpass 0.805%, whereas in cefotaxime samples, the SD was 0.154 while the RSD% did not go over 2.376%. Calculations made with the Ilkovic-Heyrovsky equation reveal the true values of the peak potential, Ep also the real number of electrons needed for the reduction of ceftriaxone and cefotaxime. The results illustrate that both ceftriaxone and cefotaxime need 5 electrons for reduction reaction. This technique has been successfully implemented in commercial ceftriaxone and cefotaxime medicines.