Evaluation of the Efficacy of Alcoholic Extracts of Echinacea Purpurea and Aloe Vera Against Methicillin-Resistant Staphylococcus Aureus Isolated from Different Infections

Abstract

The study consisted of 100 samples (swabs of burns, wounds, skin infections, and tonsillitis) collected from Samarra Hospital and Azadi Teaching Hospital in Kirkuk from 11/5/2021 to 2/5/2022. According to the phenotypic, cultural and biochemical characteristics, 55 isolates (55%) were identified as S.aureus, and the methicillin bio-disk diffusion method was used to diagnose MRSA, and the results showed that 20 isolates (36%) were MRSA. The study included testing the sensitivity of 20 isolates of MRSA bacteria to ten types of antibiotics: Methicillin, Ampicillin, Erythromycin, Oxacillin, Tetracyclin, Rifampicin, Gentamycin, Clindamycin, Ciprofloxacin, Vancomgcin). Methicillin (100%), Ampicillin (70%) and Erythromycin (70%), followed by the antibiotic Oxacillin, Tetracyclin, Rifampicin, Gentamycin, Clindamycin, Ciprofloxacin (65)% (65)%) (50%)( 45) (%) (45%) (35)%), while the lowest percentage of the antibiotic Vancomgcin was (10%) The inhibitory effect of the alcoholic ethanolic extract of Aloe Vera and Echinacea purpurea plants on MRSA bacteria was studied, and the results showed that Aloe vera extract gave an average inhibition diameter of 11.9 mm, while Echinacea purpurea extract gave 10.9 mm, and the treatment with a concentration of 250 mg/ml was superior and gave an average diameter of inhibition of 13.1 mm. The treatment of Aloe Vera at a concentration of 250 mg/ml showed the highest mean with a diameter of 14.8 mm. The Minimum inhibitory concentration (MIC) was determined for the extracts, and the MIC value varied according to the concentration and type of the extract and the source of infection.