Immunological and Physiological Study of Patients with Psychological Stress

Abstract

Background: Stress was associated with certain psychological diseases such as Alzheimer's, Schizophrenia and Major Depression diseases. Objectives: The objective of the current study was to assess the levels of some immunological parameters ( IL-1β) and (GAD 65) and physiological parameters serotonin and Dopamine Materials and methods: Five ml of blood were collected from patients and apparently healthy control(AHC) groups , by using disposable syringe then transported to a plain tube and left to clot at room temperature (20-25°C) for 15 min. The coagulated blood was centrifuged at 2500 rpm for 15 min; and then, serum was collected and distributed into aliquots of 0.25 ml in Eppendorf tubes for immunological and physiological tests. Results: Results of immunological parameters showed that there are a significant high(p≤0.05) in the levels of IL-1β in each patients groups in comparison with AHC group. Also , the data appeared that there are a significant high (p≤0.05) in GAD65 levels in each patients in comparison with AHC group. The current results about physiological tests appeared that there are significant low (p≤0.05) in serotonin and dopamine hormones in all patients groups compared with AHC group. Conclusion: From the present study concluded that there was inverse relationship in psychological stress diseases (Alzheimer, Schizophrenia and depression diseases) between immunological parameters (IL-1β and GAD65) and physiological parameters (Serotonin and dopamine hormones).