Title: Chemometric Assisted UV-Spectrophotometric And RP-HPLC Method for Estimation of Domperidone and Esomeprazole in Pharmaceutical Formulation

Abstract

The chemometric approaches were used widely as statistical tool for various analytical methods for determination of drugs in their dosage form. The chemometric methods (linear regression and inverse least square) were applied to obtained analytical data to estimate and evaluate the accurate and reliable results. For simultaneous determination of Domperidone and Esomeprazole by UV-Spectroscopy method the absorbance was taken at two wavelengths 284 nm and 301 nm λ max of Domperidone and Esomeprazole respectively. The concentration range of Domperidone and Esomeprazole was 5-30 µg/ml and 6.6-40 µg/ml which obey beer’s law. RP-HPLC method was developed for the simultaneous determination of Esomeprazole and Domperidone in the tablet dosage form by using gradient mobile phase consisting of Acetate-buffer: acetonitrile: methanol (55: 35: 10) aid of UV detector at 290 nm. For Esomeprazole and Domperidone, respectively the percentage recoveries were found compared UV spectroscopy method, HPLC method and linear regression applied for HPLC method to determine the p-value from one-way ANOVA and that shows there was no significant difference in assay between the to be 99.758% ± 0.2396% and 99.949% ± 0.2156%. Moreover, Inverse least square applied to ILS applied UV-Spectroscopy method, RP-HPLC method & Linear regression applied for HPLC method at the significant level of 0.05%.