Antimicrobial Efficacy of Nano Paint for Die Stone Dental Cast Disinfection

Abstract

Aim: The aim of this study is to evaluate and analyse the antimicrobial activity of nanopaint immersed die stone, where the nanoparticle used is silver nanoparticle incorporated with green tea and neem. Materials and methods: First, a concentrated solution of neem was prepared using neem extract powder. Similarly, in the same concentration, a green tea solution was also prepared in another beaker. Both of these were filtered, mixed and concentrated again. Separately, a solution of silver nitrate was prepared. 70 mL of the concentrated silver nitrate solution was mixed with 30 mL of the concentrated mixture of the plant extracts. The beaker was then put in a mechanical stirrer. The beaker was then put in the magnetic shaker for 24 hours. The solution was then put into centrifuge tubes and centrifuged for 10 minutes. The supernatant was removed, and the nanoparticle concentrated solution was collected in one test tube. This is the end result of the nanopaint. Die stone samples were tested for two most commonly found bacteria in the oral cavity (Streptococcus mutans and Lactobacillus). These were incubated for 24 hours, and the results were observed. Results and Discussion: The zones of inhibition after 24 hours of incubation were observed and the die stone samples which were freshly dipped in the nanopaint, were found to have the maximum activity in comparison to the dipped and dried sample and the control sample. Independent t test was conducted and the P values are statistically significant (P value < 0.05). The mean values for freshly dipped nanopaint samples (19 ± 1.0 for Lactobacillus and 15.33 ± 1.155 for S. mutans) were higher than dried samples ( 11 ±1.0 for Lactobacillus and 10 ± 1.0 for S. mutans), for both bacteria tested against them. This proves that even though both nanopaint groups have good antimicrobial property, the freshly dipped nanopaint is more effective. Conclusion: Die stone immersed in nanopaint, where the nanoparticle is silver nanoparticle incorporated with green tea and neem, shows good antimicrobial properties which will help in reducing the spread of infection in patients through dentists.