Antidiabetic and Cytotoxic Effect of Hexane Extract in Mucuna Pruriens

Abstract

Introduction: Mucuna pruriens Linn. is a common Indian medicinal plant that has long been used in traditional Ayurvedic Indian medicine for diseases like parkinsonism. This herb is pharmacologically investigated for various activities such as antidiabetic, antineoplastic, antiepileptic, antimicrobial, etc. A wide variety of phytochemical components have been isolated from this herb.Aim: The study was to determine the anti-diabetic and cytotoxic effects of hexane extract on Mucuna Pruriens. Materials and methods: Mucuna pruriens powder was weighed in a conical flask with distilled water and hexane solution added. Both of the flasks were covered with foil and kept in a shaker for 2 days and solution was added to both separate test tubes. The antidiabetic agent was added to each of the test tubes and heated. Using the spectrophotometer, a reading of both aqueous and hexane solutions was observed. Brine shrimp eggs are kept for two days to mature. In a 6 well ELISA plate artificial seawater was added to each of the 6 wells, 10 shrimps were added to the wells. The estimated volume of hexane extract is added to the brine shrimps with the help of micro-drippers. The setup is kept undisturbed for 24 hours, the number of shrimps that are alive on the first and second day is counted and tabulated. Correlation analysis is done in SPSS.Results: Mucuna Pruriens has higher inhibition and a positive correlation which produces antidiabetic efficacy. The viability of Nauplii, analyzed for different concentrations after 24hr was found, all of them were alive and after 48hr 20,40,80micro microliter, all the nauplii were aliveConclusion: The study establishes the in vitro antidiabetic activity of Mucuna Pruriens as evidenced from the hexane extract, showing a potential source for newer antidiabetic treatment.