Efficacy of Topical Hemocoagulase in improving the characteristics of Surgical Wounds: In Vitro Study

Abstract

Wound Healing Is a Complex Process Involving A Series Of Overlapping Events. Hemocoagulase Is A hemostatic agent that is recently studied for its wound healing property. Though its role in secondary wound healing has been researched, its role in primary wound healing is not studied yet. This in-vitro study aims to evaluate the efficacy of topical hemocoagulase in improving the healing of surgical wounds. This study included 6 healthy male or female (total of 12 sites) rodents weighing at least 160 grams. Under sterile aseptic conditions, the rodents were anesthetized, and a 1 cm length vertical skin incision was made on the right and left side of the vertebral column, exposing the fascia and muscles underneath. Topical hemocoagulase (test) was used on the right side of the rodent while the left side served as control. The rodents were sacrificed on postoperative days 3 and 7, and the control and test sites were excised and sent for histological analysis. The outcomes assessed were histological wound healing parameters with the wound healing scale which includes the amount of granulation tissue, inflammatory infiltrate, collagen fiber orientation, the pattern of collagen, amount of early collagen, and amount of mature collagen. Our study results showed no difference in the wound healing parameters between the test and control groups. Topical hemocoagulase, though proven to play a role in improving secondary wound healing, has no significant role in primary wound healing among the rodent species.