Physiological effects and Molecular characterization of visfatin gene polymorphism in patients with beta-thalassemia major

Abstract

Beta thalassemia major (βTM) represents the most severe form of beta-thalassemia that is recessively inherited. The study started from January 2021 to October 2021 by collecting blood samples from seventy-five (75) transfusion-dependent β-thalassemia majors. , 38 males and 37 females were registered at the center for thalassemia and inherited blood disorders, Shaheed Hemin teaching hospital, Sulaymaniyah, Kurdistan Iraq. Patients were divided into three groups. Each group included 25 patients whose ages ranged from (2 - 10), (11 - 20), and (21 - 30) years, respectively. And 15 healthy subjects. Assessments of serum levels of visfatin. Restriction Fragment Length Polymorphism (RFLP) polymerase chain reaction (PCR) was employed to detect the single nucleotide polymorphism (SNP) in visfatin. Three genotypes (CC, CT, and TT) were detected in the rs61330082 locus of visfatin, and the differences in the frequency distribution of these genotypes were significant in the two groups (p ≤ 0.001). The TT genotype was associated with a reduction in visfatin levels