Genotyping Detection of Giardia Lamblia from Human and Animal Feces Samples In Wasit Province, Iraq

Abstract

Background: Giardia lamblia, also defined as duodenalis or intestinalis, is a zoonotic enteroparasite that has a short life cycle and two active trophozoite and cystic types, both of these parasites can be identified in the stool of hosts and can be detected microscopicallyMethods: using microscopic examination of samples stained with iodine firstly, while the second one included determining the genotypes of the parasite-positive samples using the Polymers Chain Reaction (PCR) technique, and using Nested PCR to detect genotypes A, B, E and F in human and animals.Results: Genotypes A, B, E, F were detected in human samples using the molecular technique Nested PCR, recorded three genotypes in human samples A,B,E,F 7/15,8/15,0/15,0/15 , results showed are 7/15, 3/9, 3/9, 0/9 with 46.66%, 33.33%, 33.33%, for genotype A, B, E while F no sample was recorded, the results of the statistical analysis showed a significant differences between the genotypes in human samples P>0.05. In Nested PCR, three genotypes A, B, E were recorded in animal 3/9, 3/9, 3/9, 0/9 33.33%, 66.66%, 50%, 0%, respectively. While no positive sample was recorded for The F genotype.Conclusions: Giardiasis in human, men having a higher then female, children higher than adults, urban higher than rural.In Nested PCR human have genotypes A, B while animal A, B, E.