An In Vitro Examination of The Anticancer Activities of Anthocyanins and Butanol Fractions Extracted from the Rose of Bombax Ceiba Plant Grown in Iraq

Abstract

As a broad and diverse genus, bombax includes plants native to diverse ecosystems throughout Asia, the Mediterranean, and southern Australia. The purpose of this research was to extract, fractionate, and quantify a few keys physiologically active components of the plant using a Soxhlet apparatus using ethyl acetate and hexane, respectively, due to the polarity differences between these solvents. The second section evaluates the butanol and anthocyanidin fractions for potential anti-cancer efficacy. This is determined by testing the extracts for anti-cancer activity on the MCF7, AMJ13 cell lines at doses ranging from 10 to 300 µg and comparing the extracts' impact to that of a positive control (vincristine) Inhibition of cancer cell proliferation was seen in a statistically significant way (p0.05) when the anthocyanidin fraction of the plant was used. A one-way analysis of variance (ANOVA) revealed that, when compared with the positive control, vincristine, the anthocyanidins fraction of bombax ceiba was more efficient in killing cancer cells than the butanol