Molecular Diagnosis for Seropositive and Negative of Toxoplasma Gondii in Aborted Women, Sheep and Goats in Erbil Province

Abstract

The overall 85 aborted women anti-T. gondii IgG immunoglobulin was detected in 27.5% (22/80) of the women, of which 22.5% (18/80) were positive for only IgG, and 5% (4/80) were positive for IgM antibodies. Furthermore, the total seropositivity of anti-T. gondii immunoglobulin among aborted sheep and goats were 15(17.65%) and 9(30%), respectively. however, IgG plus IgM antibodies were found in (23.64%, 3.64%) and (26.67%, 3.33%), respectively. The present study showed that 8 of 165 blood and placental tissue samples were found to be positive for both IgG Cobas e 411 assay and conventional PCR. On the other hand, two blood and tissue specimens were positive for IgM and conventional PCR positive cases, and the remaining five cases of IgM were seronegative, and the remaining IgG cases were seronegative. Conventional PCR detected 12 positive cases from placental tissue that could not be detected by Cobas e 411 assays against IgM and IgG. According to molecular analysis, the specimens examined in this study were shown to share a greater 99.9%–100% agreement with sequences of T. gondii (KX270387 and MK704513) in Erbil province. The information is helpful for educational authorities responsible for designing and implementing effective measures to prevent disease.