Effect of pyridine on the histological structure of adult male rabbit’s testes Orectolagus cuniculus

Abstract

The current study aimed to identify the effect of pyridine on the histological structure of the testis in rabbits. The study included 15 rabbits, with different ages ranging between 6-9 months and with different weights of 1.100 -1.653 kg. The animals were divided into three groups, and each group contained 5 rabbits. The animals of each group were placed independently and were as follows: the first group was the control group, and the second and third group were the experimental groups and included 5 rabbits for each group. The animals of the three groups were injected with pyridine subcutaneously at a concentration of (100,200) μl of pyridine / kg of body weight for a period of time 30 days with one concentration for each group. After the completion of the experiment, the animals were sacrificed, dissected and neutered, and their tissue sections were prepared . The results of the current study showed clear changes in the weights of rabbits treated with pyridine, as the weights of all experimental group animals decreased, and this decrease was affected by the increase in concentration, reaching between (0.850-1.400) kg. The treatment with pyridine also led to clear pathological changes in the histological structure of the testis, as it led to a change in the thickness of the wall of the seminiferous tubes and their shrinkage, as their shape became wavy and irregular, and atrophy in some seminiferous tubules, and the germ cells had shedding and depletion and their collection in the lumen of the seminiferous tubules and dissolution in some Sertoli cells. It was also found that the sperm cells had separated, the epithelial layer had separated from the basement membrane, and hemorrhage had occurred in some areas. In addition, Leydig cell slackening, degeneration, and increased interstitial space occur. In the study, it was also found that the seminiferous tubules ruptured, the Sertoli cells degeneration, and the cells of the basement membrane were deformed and aggregation in the lumen of the seminiferous tubules. Also, there was an expansion in the diameter of the seminal tubules, which reached (50.2 and 54.6) micrometers, respectively, compared to the control group, which amounted to (45.8) micrometers .