Assessment of Preptin as Predict Marker for Complication in Thalassemia Major

Abstract

Hemoglobin is made up of chains of protein called globin chains, which are broken down by genetic abnormalities that cause anemia. In many parts of the world, thalassemia is a serious public health problem [1]. It's also known as Mediterranean anemia. This condition is accompanied by several biochemical alterations in the blood. Some biochemical indicators of thalassemia patients were examined and compared to a healthy control group in this study. Serum iron, ferritin, TIBC, transferrin, transferrin saturation percentage, Preptin, insulin, and lipid profile are among these markers. Preptin, iron, lipid profile, blood sugar level, and insulin resistance in the serum of patients with thalassemia were the primary objectives of this study, as well as the association between cytokine(preptin), lipid profile, blood sugar level (and insulin resistance). Material and Methods: Fifty-eight Iraqi thalassemia major patients were participated in the current investigation. They were between the ages of 1 and 28. During the months of August 2018–January 2019, these patients were registered as thalassemic patients at the "Thallassemia Unit" at the "AL-Zahra'a Teaching Hospital" in Najaf, Iraq. Thirty otherwise healthy individuals were used as a control group. Their ages corresponded to those of the patients. None of these participants were anemic or suffered from any clear systemic or chronic disorders. The glucose levels, lipid profile, TIBC, and IRON concentrations were determined using a colorimetric technique. The levels of ferritin, insulin, preptin, salusin a, and salusin β were evaluated and quantified using the ELISA method. Special formulae were used to determine the Body Mass Index and Homeostatic model assessment of insulin resistance (HOMA-IR) and HOMAβ. Results: The investigation observed a substantial rise in blood iron, ferritin, TS percent, FBG, Insulin, TG, HOMA-IR, and preptin in individuals with Thalassemia Major when compared to the healthy group, but a considerably lower level in BMI, UIBC, Total cholesterol, HDL-C, LDL-C, and HOMA- β, (p›0.001). The impacts of age, TIBC, Transferrin, and VLDL-C were not significant. Conclusions: The current study found that serum preptin, a newly bioactive peptide with potent hemodynamic activities, can be used to detect the onset and progression of heart disease and renal disease, and may be a novel therapeutic candidate for the treatment of Major Thalassemia patients, particularly the cardiovascular system. Insulin resistance has a function in the progression of diabetes in people with thalassaemia.