Evaluation of the efficacy of extracts of two types of Mollusca to control the eggs of the Greater wax moth Galleria mellonella (L.) (lepidoptera: pyralidae)

Abstract

The extracts of two types of molluscs were used, one of which was prepared in the laboratory represented by the ethyl alcohol extract of the soft tissues of the oyster Psudontopsis euphraticus (Burginat, 1852) and the aqueous extracts of its shells and their mixture under concentrations (10, 20, 30 and 40) mg.ml^-1, and conducted GC-MS analyzes of soft tissues and shells, and the analyzes of oxides and minerals by X-ray fluorescence technology of the shell extract. The other one is a commercial preparation which is a protease enzyme of Achatina fulica "White Jade" snail under concentrations (2.5, 5, 7.5 and 10) mg.ml^-1, to find out the effect of the two species in controlling the egg stages of the greater wax moth Galleria mellonella (L.) and to compare the results, a new insecticide was used to control Lepidoptera insects known as Belt_480SC (Flubendiamide) at concentrations (0.1, 0.2, 0.3 and 0.4) ml.L^-1. The soft-tissue alcoholic extract and aqueous extract of shells and their mixture were significantly superior in killing the eggs of the greater wax moth by (100%) at the highest concentration of 40 mg.ml^-1 for the two extracts and at the concentrations of 30 and 40 mg.ml^-1 for the mixture, compared to a lower mortality rate of which it reached (86.66%) for the protease at a concentration of 10 mg.ml^-1 and (90.66)% for Belt_480SC at a concentration of 0.4 ml.L^-1, and the relationship was positive between the concentrations used for the treatments and the mortality rates of eggs.