Phenotypic And Molecular Characterization of Extended- Spectrum Β-Lactamase Producer Serratia Marcesence Isolated from Surgical Wound Infections

Abstract

Introduction: Serratia marcesence is a one type of Extended-Spectrum Beta-Lactamases (ESBLs) are the major cause of resistance to beta-lactam antibiotics such as amoxicillin, cefixime, cefotaxime, ceftazidime, ceftriaxone, piperacillin tetracycline and trimethoprim-sulfa. CTX-M and SHV but not TEM genes were present in Serratia marcesence resist pathogenic form. Aim: To determine the Phenotypic and molecular characterization of Extended- spectrum β-lactamase producer Serratia marcesence isolated from surgical wound infections by detection three genes resist to different types of antibiotics. Methodology: The present study was carried out from June 2020 to June 2022 in the department of pharmacy, microbiology lab, Al-noor University College. Eighty-six 86 out of 158 wound swabs samples were extended- spectrum β-lactamase producer Serratia marcesence. The isolates were collected from patients suffering from contaminated wound surgery and studied at Al-Noor University collage laboratories by using morphological, culture, manual biochemical identification method using API 20E strip test and molecular identification by PCR-based technique. Results: All patients had signs of wound contamination like redness around wound, swelling of wound area, pus and fluid drainage from the incision area after surgery. ESBL genes were detected by PCR method among eighty-six (54.4%) swabs which contain pathogenic Serratia marcescens bacteria and phenotypically confirmed ESBL producers. The rest seventy-two (45.5%) phenotypically confirmed non-ESBL producers. The genes that interested in this study were, bla TEM (0%), followed by bla SHV (10.4%), and bla CTX-M (89.5%).The patterns of antimicrobial resistance for isolates under study showed susceptible to amoxicillin and meropenem while they resist to, Ceftazidime, Ceftriaxone, and Gentamycin Conclusion: In the present study, it was concluded that antimicrobial drugs such as Amoxicillin, Amikacin and meropenem should be highly recommended for the treatment of pathogenic S. marcescens resist strains as appropriate choice; and definitive identification of ESBL genes by molecular detection is the best and rapid diagnosing technique which is the key to control and management the surgical wound infection.